Chemotaxis of iplA- cells is impaired in the intracellular presence of a Ca2+-buffer. Wild type and mutant amoebae were loaded with Fura2-dextran and their ability to protrude pseudopods towards a cAMP-filled glass capillary was compared to that of untreated cells. In both strains the presence of the chelator in the cytosol led to a decrease in the fraction of cells extending pseudopods and migrating towards the cAMP source.
Schaloske et al. BMC Cell Biology 2005 6:13 doi:10.1186/1471-2121-6-13