General morphology of wild type and iplA- cells under control conditions (A, B), in the presence of 10 mM EGTA for 60 min (C, D) or in the presence of 10 mM CaCl2 for 80 min (E, F). In H5-buffer or in the presence of 10 mM CaCl2 the morphology was not significantly different between wild type and mutant amoebae. However, in the presence of EGTA the cells of both strains were rounded. Photographs were taken at t5. Basal motility under these conditions can be viewed in the accompanying movies.
Schaloske et al. BMC Cell Biology 2005 6:13 doi:10.1186/1471-2121-6-13