Measurement of cAMP activated [Ca2+]i-transients in wild type and mutant amoebae in the cytosolic presence of IBMX. IBMX led to an elevation of basal [Ca2+]i. Upon stimulation with 1 μM cAMP in the absence of external CaCl2 a [Ca2+]i-transient was observed in both, mutant and wild type amoebae (mean ± s.e.m.).
Lusche et al. BMC Cell Biology 2005 6:12 doi:10.1186/1471-2121-6-12