Co-immunoprecipitation of Scar3 and Abi1 from mouse brain extract. Protein G beads were used to precipitate Abi1 from mouse brain extracts in the presence or absence of (A) anti-Abi1 or an unrelated control antibody or (B) anti-Scar3 or an unrelated control antibody. (A) The beads fractions were probed with anti-Abi1, anti-Scar/WAVE1 and anti-Scar/WAVE3. Anti-Abi1 immunoblotting confirmed precipitation of Abi1 only with specific antibody and immunoblotting with anti-Scar/WAVE1 shows association with a known binding partner. Immunoblotting with anti-Scar/WAVE3 revealed Scar/WAVE3 to also be present in Abi1 immunoprecipitates. Anti-Abi1 pulls down both Scar1 and Scar3 together with Abi1. (B) Beads fractions from anti-Scar/WAVE3 immunoprecipitates were probed with both anti-Scar/WAVE3 and anti-Abi1. Both Scar3 and Abi1 are detected in anti-Scar/WAVE3 immunoprecipitates, but not with controls.
Stovold et al. BMC Cell Biology 2005 6:11 doi:10.1186/1471-2121-6-11