Scrib interacts with LPP in mammalian cells. pM-bait- and pSNATCH-prey-constructs were cotransfected into 293 cells in the combination indicated, together with a GAL4-regulated luciferase reporter and a CMV-β-galactosidase internal control. Cell lysates were assayed for luciferase activity 18–24 hours after transfection. Relative luciferase activity is reported as the average of three independent duplo experiments (with standard error).
Petit et al. BMC Cell Biology 2005 6:1 doi:10.1186/1471-2121-6-1