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Open Access Methodology article

Protein-trap version 2.1: screening for expressed proteins in mammalian cells based on their localizations.

Olga O Sineshchekova, Toshimitsu Kawate, Oleksandr V Vdovychenko and Thomas N Sato*

Author Affiliations

The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390, USA

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BMC Cell Biology 2004, 5:8  doi:10.1186/1471-2121-5-8

Published: 2 February 2004

Abstract

Background

"Protein-trap" is a method that allows epitope-tagging of endogenous proteins. This method allows for the identification of endogenously expressed proteins that exhibit specific localization of interest. This method has been recently reported for its application in the study of Drosophila development by using a relatively large epitope, green-fluorescent-protein (GFP).

Result

Herein, we report a new "protein-trap" vector for mammalian cells. This new method utilizes a much smaller epitope-tag and also allows for drug-selection prior to the epitope-tagging. Pre-selection by drug resulted in the highly efficient protein-trapping frequency.

Conclusion

The "protein-trap" method based on this new vector is expected to serve as a complimentary approach to the previously reported GFP-based method.