Effects of LipofectAMINE 2000 reagent on insulin-stimulated glucose transport in adipocytes and muscle cells. A) 3T3L1 preadipocytes were differentiated to adipocytes and treated with and without LF2000 as described in the methods section. Insulin-stimulated glucose transport was measured in replicates of six for each treatment. Data are from a single experiment which is representative of five such experiments that gave similar results. B) Rat L6 myocytes were differentiated to myotubes as described in the methods section and treated with LF2000 as above. Glucose transport was measured in a similar manner to that for adipocytes. Data are from one experiment that has been repeated at least four times with similar results. C) SHSY5Y neuroblastoma cells were cultured and stimulated with insulin as described in the methods section and glucose transport measured as above. Data are from one experiment performed in sextuplet, which has been repeated two further times. **p < 0.005 with respect to no-LF2000 control; ***p < 0.0005 with respect to no-LF2000 control.
Pramfalk et al. BMC Cell Biology 2004 5:7 doi:10.1186/1471-2121-5-7