Figure 7.

Chromatin intensity analysis showing the preprocessing steps for the Hoechst 33342 images: a) unprocessed original image; b) Gaussian smoothing; c) image classified into 8 regions of grey values; d) localization of particles in the cell nucleus (red); e) measuring the mean grey value intensity around the center of mass for each individual vimentin particle (see Methods for a complete description).

Bacher et al. BMC Cell Biology 2004 5:45   doi:10.1186/1471-2121-5-45
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