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Resolution: standard / high Figure 4.
Localization of VHA-subunits in plant cells, using anti-VHA-A, anti-VHA-E and anti-VHA-aNterm-antibody Confocal images represent single images of isolated maize root cells. Immuno-staining
was performed on maize root cells. Secondary fluorescently labelled antibodies used
were anti-rabbit-FITC (A-F) or anti-guineapig (Cy5). Images were colour coded with
Adobe Photoshop. Scalebars are 10 μm. Labelling of tonoplast membranes with anti VHA-A
(A to C) and anti VHA-E (D-F) in root cells of increasing age, i.e. non-vacuolized
to vacuolized (left to right). Anti-VHA-aN-term labelling of root cells of increasing age (G-I). Note that the staining pattern of
VHA-aN-term is distinct from the tonoplast labelling with VHA-A and VHA-E in all cases.
Kluge et al. BMC Cell Biology 2004 5:29 doi:10.1186/1471-2121-5-29 |
