The effects of SSW on microtubules. (A, B) Fluorescence images of microtubules labeled with an antibody to tubulin. (A) Control cells showed a characteristic, brightly labeled microtubule organizing center (MTOC=centrosome) with microtubules radiating outward throughout the cytoplasm. (B) SSW-treated cells showed a much less orderly extension of the tubules, and the MTOC were much less organized than in the control cells. (C) Immunoblot analysis showed an increase in tubulin after smoke treatment. For equal loading of protein in the immunoblots, please refer to grp78 blots in Fig. 4C; the same membrane was used to reprobe for the protein shown in this figure.
Wong et al. BMC Cell Biology 2004 5:13 doi:10.1186/1471-2121-5-13