Figure 2.

Real time qPCR and immunoassays Relative amount of cyclin A, E and topoisomerase II α mRNA determined by running real time quantitative PCR on reverse transcribed total RNA, extracted from HTB-125, MCF-7 and MDA-231 cells in G1-phase, which were assorted by centrifugal elutriation. The algorithm, chosen for calculating the relative amount of target mRNA gives values without any units of measurement. The detection range of the instrument is in our case five orders of magnitude. For the protein determination we calculated the percentage of positively stained G1-phase cells that were assorted by flow cytometry.