Cell cycle assay of induced cell lines. Cell lines expressing GFP-BLM alleles described previously  were induced for five days with 1 μg/ml doxycycline. Cell cultures were harvested, fixed and stained with PI. Relative DNA content was measured by FACS analysis. A. Comparison of DNA content profiles of the cell lines expressing the normal and N-terminal deletion alleles. B. Comparison of DNA content profiles of the cell lines expressing the normal, helicase and C-terminal mutant alleles.
Schawalder et al. BMC Cell Biology 2003 4:15 doi:10.1186/1471-2121-4-15