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The effect of IGF-1 upon the in vivo fertilized embryos examined for apoptosis and necrosis with fluorescent microscopic examination of PI and Annexin V staining. |
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| Study methods |
Propidium iodide and Annexin V --FITC studies |
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| Culture medium |
Total n |
With PI-positive cells * |
With Annexin V-positive and PI-negative cells ** |
With Annexin V-positive or PI-positive cells *** |
Without Annexin V- and PI-positive cells **** |
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| kSOM/AA with IGF-1 |
106 |
5(4.7%) All are arrested embryos |
5(8.5%) All are arrested embryos |
10(9.4%)# |
96(86.8%)# All blastocysts and 2 arrested embryos |
| kSOM/AA without IGF-1 |
83 |
7(8.4%) All are arrested embryos |
13(15.6%) Arrested embryos and five blastocysts |
20(24.1%)# |
63(75.9%)# All are blastocysts |
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IGF-1: 100 ng/ml. The results were pooled from 5 repeats of experiments. # P < 0.05 * With necrotic cells; at least one PI-positive cell in the embryo, no matter if Annexin V-stained cells are present or not. ** With apoptotic cells; at least one Annexin V-positive cell in the embryo, but no PI-positive cells observed. *** With apoptotic cells or necrotic cells. **** Normal without apoptotic cells or necrotic cells. | |||||
Lin et al. BMC Cell Biology 2003 4:14 doi:10.1186/1471-2121-4-14 |
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