Table 1

Summary of antibodies, their known MyHC epitopes and epithelial reactivity.

Antibody

Muscle reactivity

Epithelial reactivity



MyHC Gene

Epitope location

Expression timing

Surface epidermis

Hair¶follicle

Gut

Non-neural retina

Bladder


A4.840

Slow/β

Rod

Embryo onwards

All

IRS/ ORS

All

+

+

HRMC§

H

HRMC

HRMC

HRMC*

R

R

R

N3.36

Neo/adult fast

N-terminal 25 aa

Fetal/ postnatal

Some basal cells

ORS

Crypt base

+

+

HRM

H

HRM

HR (not M)

HR (not M)

R

R

R

N2.261

Slow/β + Fast IIA

N-terminal 25 aa

Neonatal onwards

Basal

-

-

+

+

HRM

H(β)

RM

HR

HR

R

R

R

A4.74

Fast IIA

Unknown

Postnatal onwards

-

IRS

-

-

-

HRM

RM

HRM

HRM

R

R

R

N1.551

Neonatal + Adult Fast

Unknown

Fetal onwards

-

Bulge? neck cell cluster

-

HRM

HRM

R

R

R

A4.951

Adult slow/β

N-terminal 25 aa

Postnatal onwards

Weak in fetus only

Bulge? neck cell cluster

-

HRM

H

RM

R

R

R

A4.1025

All known

20 kd / 25 kd junction/ ATP site

Embryo onwards

-

-

-

-

-

HRMCDZX

H

HRMCDZX

HRM

HRM

R

R

R


Note that three antibodies (N3.36, N2.261 and A4.951) that detect MyHC-like antigens in epithelia have MyHC epitopes in the N terminus of the S1 fragment of the head. X-ray crystallography does not reveal a coiled coil (or any other conserved structure) in this region. §Letters under each entry indicate the species in which reactivity is observed: H human, R rat, M mouse, C chick, D Drosophila, Z zebrafish, X Xenopus. Absence of a species indicates data not available unless specifically noted otherwise. ¶IRS inner root sheath, ORS Outer root sheath, * in feather germ.

Jazwinska et al. BMC Cell Biology 2003 4:10   doi:10.1186/1471-2121-4-10

Open Data