Cellular and sub-cellular localisation of A4.840-antigen. Immunofluorescent detection of A4.840 (A,C,E,F red; G,I green), A4.74 (B,D, green), and desmoplakin (E,F, green; H,J, red) in rat epidermis. A-D. A4.840-epitope is distributed at the cell periphery of most cells of trypsinised epidermis (A,B a hair follicle bulb in high mag transverse section; C,D surface and hair follicle epidermis in low mag longitudinal section). The A4.74-epitope is in fibrillar structures within the outermost layer of A4.840-reactive cells in the root sheath (arrows, B,D). E,F. Confocal microscopy using A4.840 (red) and anti-desmoplakin (green) on cryosections of P7 rat back skin. Desmoplakin co-localises with A4.840-antigen labelling at the surface of cells in many layers of the root sheath of the hair follicle (E). At higher magnification, desmoplakin and A4.840-antigen co-localise in a punctate array at cell borders in surface epidermis (F). G-J. Intact pieces of P7 rat epidermis were stained for A4.840 (green G,I) and desmoplakin (red H,J). A network of cell surface staining is detected with both antigens after permeabilisation with 1% Triton X100 (G,H arrows), but not without such permeabilisation, when only background fluorescence was detected (I,J arrows).
Jazwinska et al. BMC Cell Biology 2003 4:10 doi:10.1186/1471-2121-4-10