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Open Access Research article

Intermediate filament-co-localized molecules with myosin heavy chain epitopes define distinct cellular domains in hair follicles and epidermis

Ania Jazwinska2, Elisabeth Ehler1 and Simon M Hughes1*

Author affiliations

1 Randall Centre, 4th floor south, New Hunt's House, Guy's Campus, King's College London, London SE1 1UL, UK

2 current address: Dept. of Cell Biology, Biozentrum, Klingelbergstr. 70, CH-4056 Basel, Switzerland

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Citation and License

BMC Cell Biology 2003, 4:10  doi:10.1186/1471-2121-4-10

Published: 4 August 2003

Abstract

Background

Proteins linking intermediate filaments to other cytoskeletal components have important functions in maintaining tissue integrity and cell shape.

Results

We found a set of monoclonal antibodies raised against specific human sarcomeric myosin heavy chain (MyHC) isoforms labels cells in distinct regions of the mammalian epidermis. The antigens co-localize with intermediate filament-containing structures. A slow MyHC-related antigen is punctate on the cell surface and co-localizes with desmoplakin at desmosomal junctions of all suprabasal epidermal layers from rat fœtal day 16 onwards, in the root sheath of the hair follicle and in intercalated disks of cardiomyocytes. A fast MyHC-related antigen occurs in cytoplasmic filaments in a subset of basal cells of skin epidermis and bulb, but not neck, of hair follicles. A fast IIA MyHC-related antigen labels filaments of a single layer of cells in hair bulb. This 230 000 Mr antigen co-purifies with keratin. No obvious candidate for any of the antigens appears in the literature.

Conclusions

We describe a set of molecules that co-localize with intermediate filament in specific cell subsets in epithelial tissues. These antigens presumably influence intermediate filament structure or function.

Keywords:
gut; heart; cardiomyocyte; intercalated disk; intermediate filaments; desmosomes.