Table 3

PMA Enhances the Cytotoxicity of CEES to RAW264.7 Macrophages (24 hours)1

Assay2

Control

LPS

PMA

CEES

LPS+CEES

PMA+CEES


PI (%)

0.0 ± 0.0 a

2.6 ± 1.03a

1.8 ± 0.96a

18.7 ± 6.23b

59.1 ± 6.63c

41.4 ± 5.0 d

MTT3

1.50 ± 0.03a

1.56 ± 0.06a

1.71 ± 0.03a

1.46 ± 0.10 b

1.07 ± 0.11c

1.36 ± 0.04 b


1LPS was used at 25 ng/ml, PMA at 50 ng/ml and CEES 500 μM. Values are means ± SD and means (in a given row) not sharing the same superscript letter are significantly different (p < 0.05). 2PI(%) is the percent of propidium iodide fluorescence over that of the control cells. 3The MTT levels are given as 575 nm absorbance units.

Stone et al. BMC Cell Biology 2003 4:1   doi:10.1186/1471-2121-4-1

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