GHFT1-5 cells were grown to subconfluence and treated for 20 hours with DMSO (white bars), 100 ng/ml nocodazole (gray bars) or 0.5 mM mimosine (speckled gray bars), both in DMSO. The amount of DNA within each cell was measured by flow cytometric quantification of the orange fluorescence emitted from propidium iodide-stained DNA. The proportion of GHFT1-5 cells containing a 2n complement of DNA (G1), a 4n complement of DNA (G2/M), and an intermediate amount of DNA (S) was determined for each treatment and presented as the mean +/- standard deviation from nine independent experiments.
Liu et al. BMC Cell Biology 2002 3:6 doi:10.1186/1471-2121-3-6