Table 1

Quantitation of myosin-II distribution during cytokinesis : Comparison with minimal force requirements.

Interphase

Spherical

Cylindrical

Dumbbell


Cell volume, μm3 a

620

620

690

720

Volume of cell cortex, μm3 a

120

120

130

160

Volume of furrow, μm3 a

-

-

40

20

Total wild type myosin-II, % total [μM]

100 [3.4]

100 [3.4]

100 [3.4]

100 [3.4]

Cytoplasmb

77 ± 2 [3.2]

79 ± 2 [3.4]

79 ± 2 [3.4]

73 ± 2 [3.2]

Total cortexb

23 ± 0.3 [4.0]

21 ± 0.5 [3.6]

21 ± 0.5 [3.8]

27 ± 0.5 [4.2]

Furrow cortexb

-

-

8.4 ± 0.2 [5.0]

5.3 ± 0.4 [6.4]

Total 3 × Ala myosin-II, % total [μM]

100 [3.4]

100 [3.4]

100 [3.4]

100 [3.4]

Cytoplasmb

74 ± 2 [3.2]

77 ± 2 [3.2]

76 ± 8 [3.2]

70 ± 4 [3.0]

Total cortexb

26 ± 0.8 [4.6]

23 ± 0.5 [4.0]

24 ± 1 [4.4]

30 ± 0.1 [4.6]

Furrow cortexb

-

-

13 ± 1 [7.6]

16 ± 1 [18]

Minimal force requirements, nNc

-

-

7.2

2.7

% of total myosin-II required for this amount of forced

-

-

12

4.5


a These quantities were calculated based upon the geometry and dimensions of the cell. b These quantities were calculated from the empirically determined concentrations derived from the ratio imaging analysis. c These quantities were calculated based upon the dimensions of the cell. d These quantities were calculate by considering the amount of minimal required force, the physical properties of myosin-II and the amount of myosin-II in the cell.

Robinson et al. BMC Cell Biology 2002 3:4   doi:10.1186/1471-2121-3-4

Open Data