Table 1

Summary of Galectin-1 Mutations

Mutation
 Yield (mg/l culture)
 Haemagglutination. Endpoint (μg/ml)
 Growth Inhibition I50 (μg/ml)
None
 8.0
 1
 5
R49G*
 0.1
 1
 nd
N47D*
 0.1
 100
 nd
A28R*
 0.3
 -
 >200
D103A
 9.2
 1
 20
P79R
 0.6
 500
 4
C131S
 0.5
 -
 13
K29M
 8.0
 1
 -
K29T
 8.2
 1
 -
D27N
 0.8
 1
 170
M1R
 10.4
 1
 -
M1R (thrombin)
 n/a
 1
 24

Yields and Biological Activities of Recombinant Galectin-1 Derivatives For each mutation in galectin-1, the yield of protein per litre of bacterial culture, the endpoint of the haemagglutination titration (in μg/ml) and the concentration giving 50% inhibition of fibroblast growth (I50, in μg/ml) are listed. The three entries marked * are for low-yield mutations, for which full analysis was not feasible. The final row contains data for the M1R mutant following thrombin cleavage and repurification.

Scott and Zhang BMC Cell Biology 2002 3:3   doi:10.1186/1471-2121-3-3