Fluorescence microscopy of S2 cells expressing wild type P0/GFP in three different layers. In all layers, green vesicles are clearly visible in the cytoplasm. In (B) and (C) the equator of the cells is matched and the green fluorescence of the membrane is visible indicating the functional incorporation of wild type P0-GFP fusion proteins. Arrows indicate wild type P0-GFP in cytosolic vesicles and at the cell surface. The nuclei are free of fluorescence in contrast to the isolated GFP fluorescence (see fig. 4).
Ekici et al. BMC Cell Biology 2002 3:29 doi:10.1186/1471-2121-3-29