Fluorescence microscopy of a S2 cell transfected with the Ala221fs mutation in fusion with GFP (vector pEXII). None of the different layers analysed by fluorescence microscopy showed a membrane insertion of P0-GFP. Fluorescent cytoplasmic vesicles (arrows) are clearly visible. The nucleus is free of fluorescence (see fig. 4).
Ekici et al. BMC Cell Biology 2002 3:29 doi:10.1186/1471-2121-3-29