MIB induces G1 cell cycle arrest and inhibition of CDK2 activity (A) LNCaP cells were grown in the absence or presence of 10 ng/ml MIB and harvested after the times indicated. Cell numbers were determined by counting in a hemacytometer. (B) LNCaP cells were maintained in the absence or presence of 10 ng/ml MIB for the indicated times, and cells were harvested for flow cytometry. (C) Cyclin E immunoprecipitates were retrieved from LNCaP cells treated with 10 ng/ml MIB for 72 h and examined for associated H1 kinase activity in vitro. The precipitated amount of cyclin E (top) and the associated kinase activity (H1-P) are shown (bottom). (D) Cell lysate was prepared from LNCaP cells maintained in the absence or presence of 10 ng/ml MIB for 72 h. Lysates were precipitated with cyclin E antibodies and immunoprecipitates were examined for co-precipitation of p27 by immunoblotting (lanes 1 and 2). The asterisk denotes the immunoglobulin heavy chains. Total cell lysates are shown in lanes 3 and 4.
Lu et al. BMC Cell Biology 2002 3:22 doi:10.1186/1471-2121-3-22