Figure 8.

Effect of pH on the fluorescence of oxidized OxyBURST Green H2HFF BSA. Oxidized OxyBURST Green H2HFF BSA (■) and 5-(and -6)-carboxyfluorescein (◆) were prepared in MES buffer solutions at various pHs, to a final concentration of 78 μg/ml and 4.2 μM respectively. The ratio of emission fluorescence at λ535 upon excitations with λ485 over λ440 (i.e. F535485)/F535440)) was then determined.

Chen BMC Cell Biology 2002 3:21   doi:10.1186/1471-2121-3-21
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