Effect of pH on the fluorescence of oxidized OxyBURST Green H2HFF BSA. Oxidized OxyBURST Green H2HFF BSA (■) and 5-(and -6)-carboxyfluorescein (◆) were prepared in MES buffer solutions at various pHs, to a final concentration of 78 μg/ml and 4.2 μM respectively. The ratio of emission fluorescence at λ535 upon excitations with λ485 over λ440 (i.e. F535(λ485)/F535 (λ440)) was then determined.
Chen BMC Cell Biology 2002 3:21 doi:10.1186/1471-2121-3-21