The effect of H2O2 and O2- on the fluorescence of LysoTracker Red DND-99. 2 μM of LysoTracker Red DND-99 was prepared in MES buffer solutions at either pH 4.5 (panel A) or pH 7.5 (panel B). H2O2 (◆) or KO2 (■) were then added to various final concentrations and the resulting reaction solutions were incubated at room temperature for 10 min. Fluorescence of the LysoTracker Red was then determined.
Chen BMC Cell Biology 2002 3:21 doi:10.1186/1471-2121-3-21