MDR1mRNA and P-gp expression in astroglial cultures. a/ RT-PCR showing MDR1mRNA. b/ Western blotting using rabbit anti-P-gp polyclonal antibody demonstrating the presence of P-gp as early as 2 div. Between 2 div and 5 div, a band is observed at about 120 kDa (lower black arrow); a doublet appears at 6 div at about 150–170 kDa (black arrows); the band corresponding to the mature form of P-gp, is expected at 170 kDa (grey arrow), and becomes increased after 15 div.
Monville et al. BMC Cell Biology 2002 3:20 doi:10.1186/1471-2121-3-20