Figure 5.

C-terminal 35 amino acids of sα_i2 do not affect GFP localization Expression vectors encoding GFP (A), GFP-sα_i235aa (B), or α_i2(1-10)-GFP (C) were transfected into HEK293 cells. 48 h after transfection, cells were fixed on coverslips, and subcellular localization was determined by fluorescence microscopy visualization of GFP.