Figure 3.

Localization of sαi2 to Golgi and ER membranesi2 was expressed in COS-7 cells. 48 h after transfection, cells on coverslips were fixed and processed for immunofluorescence. Co-localization of sαi2 (A) and the Golgi protein, β-COP (B), was visualized after incubation of cells with the EE antibody and a rabbit polyclonal anti-β-COP antibody followed by Alexa 594 anti-mouse and Alexa 488 anti-rabbit antibodies. Co-localization of sαi2 (C) and the endoplasmic reticulum protein, protein disulfide isomerase (PDI) (D), was visualized after incubation of cells with the EE antibody and a rabbit polyclonal anti-PDI antibody followed by Alexa 594 anti-mouse and Alexa 488 anti-rabbit antibodies.

Wedegaertner BMC Cell Biology 2002 3:12   doi:10.1186/1471-2121-3-12
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