Release of annexin V-binding membrane microparticles and the level of cell apoptosis in HUVEC stimulated with camptothecin. Confluent HUVEC at 48 hrs in culture were incubated with 0.5 % DMSO or with 500 nM or 5 μM CPT in medium. After indicated time periods supernatant from cell culture was harvested and annexin V-FITC-positive MP were counted (A). Adherent cells were harvested and portions of cells binding annexin V (propidium iodide negative) (B) and portions of TUNEL-positive cells (C) were evaluated. Means of 4 experiments ± SD are presented, * = p < 0.05 vs. DMSO (ANOVA).
Šimák et al. BMC Cell Biology 2002 3:11 doi:10.1186/1471-2121-3-11