Flow cytometry measurements of Tag and DNA. This is an example of the cytometric data collected for this study. P0-3D tkT#5 cells were immunofluorescently labeled with PAB 416 (anti-Tag) or IgG2a (isotype control) and FITC-conjugated goat anti-mouse secondary antibody. DNA was stained with propidium diiodide. Mean linear immunofluorescence of the G1 sub-population for the isotype control (Fb) was subtracted from that of the Tag-stained sample (Ft) to generate the measurement of Tag expression (Fs).
Frisa and Jacobberger BMC Cell Biology 2002 3:10 doi:10.1186/1471-2121-3-10