Table 1

Effect of neomycin sulfate and propranolol on the L-T4-induced DAG formation in [14]oleic acid pre-labeled hepatocytes.

Time,

Additions

Control

L-thyroxine

% of control

min


% total14C-lipids

NaOH

0.96 ± 0.11

1.34 ± 0.09*

140

0.25

NaOH+neomycin sulfate

1.43 ± 0.37

1.38 ± 0.04

96.7

NaOH

1.19 ± 0.06

2.02 ± 0.14*

170

2.0

NaOH+neomycin sulfate

1.08 ± 0.11

1.43 ± 0.09

132

NaOH+ propranolol

1.97 ± 0.21

1.77 ± 0.15

90


[14C]oleic acid labeled hepatocytes were pre-incubated for 15 min prior to NaOH (l00 nM) or LT4 (l0 nM) addition or pre-incubated for 15 min with neomycin sulfate (1.5 mM) or propranolol (100 nM) prior to stimulation with L-T4 (l0 nM).The data are mean ± S.E. of four separate experiments. * P < 0.05 vs. control.

Kavok et al. BMC Cell Biology 2001 2:5   doi:10.1186/1471-2121-2-5

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