Table 1

Distance from immunogold particles to the nearest contact site in tomographic reconstructions of liver mitochondria

Labeling type on Mitochondrion

Mean distance experimental (nm)a

Mean distance random (nm)a,b

Number of contact sites

Number of immunogold particles


WT tBid 1

53 ± 27

122 ± 56

39

23

GE tBid 1c

30 ± 14

70 ± 28

62

37

GE tBid 2

29 ± 12

129 ± 76

13

5

GE tBid 3

31 ± 6

77 ± 25

17

7

GE tBid 4

23 ± 9

104 ± 48

19

7


aMean ± standard deviation. Number of nearest neighbor measurements = number of immunogold particles. bPositions corresponding to gold particles were randomly placed around the outer membrane for each mitochondrial tomographic reconstruction according to the number of immunogold particles found in each mitochondrion (last column) and the Euclidean distance from these loci to the nearest empirical contact site was measured. cNote that "WT tBid 1" and "GE tBid 1" have larger number of contact sites and gold particles because more of the mitochondrial volume was found in these particular thick sections. The other three mitochondria had smaller portions sliced in their respective sections.

Lutter et al. BMC Cell Biology 2001 2:22   doi:10.1186/1471-2121-2-22

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