Table 2

Changes of mitochondrial membrane potential, assessed by flow cytometry using JC-1 red/green fluorescence ratio in in control and Scott cells after 48 or 72 h of apoptosis induction by calcium ionophore A23187 (200 nM) or SKF 96365 (10 μM) in the absence and presence of the caspase inhibitor z-VAD.fmk (50 μM). Values are the mean ± SEM of 4 independent experiments.

CELLS

No treatment

A23187 (200 nM)

SKF 96365 (10 μM)

zVAD + A23187

zVAD + SKF 96365


Control (48 h)

1.37 ± 0.24

1.05 ± 0.16

0.85 ± 0.11*

1.16 ± 0.03

1.01 ± 0.09

Scott (48 h)

1.25 ± 0.20

1.01 ± 0.17

0.96 ± 0.20*

1.11 ± 0.08

1.20 ± 0.12

Control (72 h)

1.26 ± 0.09

1.1± 0.16

0.89 ± 0.06*

0.97 ± 0.03

0.88 ± 0.04*

Scott (72 h)

1.55 ± 0.12

1.02 ± 0.22*

1.04 ± 0.26*

0.97 ± 0.16*

0.89 ± 0.21*


*P < 0.05 versus the corresponding values obtained with non-treated cells.

Martínez and Freyssinet BMC Cell Biology 2001 2:20   doi:10.1186/1471-2121-2-20

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