Table 2

Sister Chromatid Assay of Transfected Cell Lines: exchange data

Mean # of

Mean # of

Cell Line

SCEs per

SCEs per

Range of

Range of

% Relative

46Cs-Tet

46Cs +Tet

SCEs -Tet

SCEs +Tet

Correction


GFP

70

65

58-96

55-75

7

GFP-BLM

40

12

30-49

10-19

70

GFP-ΔN1

56

20

32-73

12-29

64

GFP-ΔN2

47

17

25-63

9-27

64

GFP-ΔN3

58

23

40-85

11-35

60

GFP-ΔN4

66

45

46-89

20-68

30

GFP-K695T

84

106

63-118

79-131

-26

GFP-ΔH1

108

95

84-159

87-142

12

GFP-ΔC1

63

89

42-101

44-205

-41

GFP-ΔC2

68

78

51-82

59-108

-15


Frequency of SCEs are shown as mean number of exchanges per 46 chromosomes (Cs) for at least 20 metaphases for each cell line without inducer and with inducer. The range of the SCEs is shown. The relative percentage correction is calculated from the ratio of the mean number of exchanges per 46 chromosomes +Tet / the mean number of exchanges per 46 chromosomes - Tet. The cell line expressing GFP alone is 0.93 and 7% correction. The GFP-BLM cell line ratio is 0.3 and 70% correction. Cell lines were induced with 1 μg/ml Tet.

Yankiwski et al. BMC Cell Biology 2001 2:11   doi:10.1186/1471-2121-2-11

Open Data