Figure 4.

Localization of GFP-BLM fusion proteins. The immunofluorescent images show representative cells from each transfected cell line. Localization of GFP or GFP-BLM fusion proteins and PML (α-PML and Donkey α-mouse Texas Red secondary) or TOPIIIα (α-TOPIIIα and Donkey α-rabbit Texas Red secondary) in DAPI-stained nuclei are shown. A. EGFP + α-PML. B. GFP-BLM + α-PML. C. GFP-BLM + α-TOPIII. D. GFP-ΔN1 + α-PML. E. GFP-ΔN2 + α-PML. F. GFP-ΔN2 + α-TOPIII. G. GFP-ΔN3 + α-PML. H. GFP-ΔN4 + α-PML. L GFP-K695T + α-PML. J.GFP-ΔH1 + α-PML K. GFP-ΔC1 + α-PML. L. GFP-ΔC2 + α-TOPIII. The parental SV40-transformed BS cell line lacks detectable BLM [10].

Yankiwski et al. BMC Cell Biology 2001 2:11   doi:10.1186/1471-2121-2-11
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