Table 1

Use of inhibitors to probe the basis for elongation of BPAEC induced by resveratrol. These experiments used passages 4-5 BPAEC cells. The cells on cover slips were incubated with inhibitors for 24 h, before 100 μM resveratrol was added and incubation was continued for an additional 40 h. An exception was quin2-AM, which was applied 1 h prior to resveratrol treatment.

Cellular

Controlª

Cells treated with 100 μM resveratrola

Component


affected

% elongated cells

% elongated cells


Control

12.90 ± 5.56

33.80 ± 7.50

Intracellular

15.80 ± 1.42

11.73 ± 1.89

[Ca2+]

PKC

22.47 ± 3.58

37.63 ± 6.17

Tyrosine kinases

9.65 ± 1.05

10.55 ± 1.05

Actin

Microfilaments

10.40 ± 2.60

12.25 ± 3.75

Actin

Microtubules

4.17 ± 4.94

8.60 ± 6.53


a Results in this table were averaged from 4 separate experiments, each performed with a different preparation of primary BPAEC's. Since growth of control cells as well as their response to resveratrol and addition of inhibitors differed between BPAEC preparations, cells showing the elongated shape in each experiment were calculated as a percent of total cells counted (ranging from 60-700). The numbers obtained in this manner were used to calculate the mean (%)± SD, as shown.

Bruder et al. BMC Cell Biology 2001 2:1   doi:10.1186/1471-2121-2-1

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