Rapamycin promoted thrombosis in rats. (A) The diagram of the DVT rat model illustrated that the iliac vein which was tied at site A was injected with rapamycin or DMSO and then tied tightly at the sites of a and b. (B) The ratio of thrombosis in rapamycin group (80%) is far more than that in DMSO (20%) group. n = 10 in each group. (C) HE staining exhibited thrombosis in the venous lumen at the time of the 40th minute. Enlarged squares indicate the platelet-derived clot appeared in the thrombus. (D) Transmission electron microscope analysis exhibited microvilli-like structure on endothelial of rapamycin treated rats compared with the smooth endothelial surface in the DMSO treated rats (TEM × 10, 000).
Jiang et al. BMC Cell Biology 2014 15:7 doi:10.1186/1471-2121-15-7