Histone H3K4Me3 modification levels in hematopoietic cells. A - Representative confocal images of fluorescence intensity of histone H3K4Me3 in CD34+ cells (CD34+), KG1 control (KG1_C), KG1 cells treated for 48 h with 4 mM PB (KG1_PB) or 25 μM RG108 (KG1_RG) and human mature neutrophils (NF) from two of three experiments showing similar results; B - 3D profile of fluorescent intensity of corresponding bottom image from (A); C - Ratios of median values of fluorescent intensities are presented. Total fluorescence intensity of each cell category (CD34+, KG1_C, KG1_PB, KG1_RG, NF) was used for computation of median values. Ratios represent fold-change (increased intensity - positive fold-change; decreased intensity - negative fold-change) of KG1_C, KG1_PB, KG1_RG, NF compared to CD34+. The Wilcoxon rank – sum test was used for statistical analysis: *, P < 0.05; **, P < 0.01; NS – no significant change. The bars represent fold enrichment of the modified histones relative to the control (CD34+ cells). Data is the mean ± SD from three independent experiments.
Navakauskienė et al. BMC Cell Biology 2014 15:4 doi:10.1186/1471-2121-15-4