Additional file 6: Figure S6.

Caspase 3/7 activity of control ER-E66D and experimental ER-MXD3 cell lines treated with either vehicle, 12 hours of 1 μM 4-OHT, 72 hours of 1 μM 4-OHT, or 6 hours of 150 μM H2O2. Treatments were staggered such that all samples were collected at the same time. Caspase 3/7 activity was measured with a Caspase-Glo 3/7 assay kit (Promega) in a 100 μl reaction volume. Specifically, 50 μl of the caspase detection reagent was substituted with 2x103 cells in 50 μl of media and incubated for 1 hour in a 96-well white walled/clear bottom plate. Subsequently, luminescence was detected using a M5 SpectraMax plate reader (Molecular Devices).

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Ngo et al. BMC Cell Biology 2014 15:30   doi:10.1186/1471-2121-15-30