Open Access Highly Accessed Research article

IRE1 inhibition perturbs the unfolded protein response in a pancreatic β-cell line expressing mutant proinsulin, but does not sensitize the cells to apoptosis

Liling Zhang1, Courtney Nosak12, Pietro Sollazzo1, Tanya Odisho12 and Allen Volchuk123*

Author Affiliations

1 Division of Advanced Diagnostics-Metabolism, Toronto General Research Institute, University Health Network, 101 College Street, TMDT 10-706, Toronto, ON, Canada

2 Department of Physiology, University of Toronto, Toronto, ON, Canada

3 Department of Biochemistry, University of Toronto, Toronto, ON, Canada

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BMC Cell Biology 2014, 15:29  doi:10.1186/1471-2121-15-29

Published: 10 July 2014

Additional files

Additional file 1: Table S1:

List of genes induced >1.5 fold by mutant proinsulin expression and mean fold-change induction compared to control cells from N = 2 independent microarray experiments. Column three is the mean fold-change induction of the same genes in the presence of the IRE1 inhibitor 4μ8c. Red: genes whose induction was not affected by 4μ8c; Blue: genes whose fold-induction was reduced by 4μ8c, but whose expression was still >1.5 fold. Green: genes whose induction in response to mutant proinsulin expression was no longer >1.5 fold in the presence of the inhibitor.

Format: XLSX Size: 17KB Download file

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Additional file 2: Table S2:

List of genes reduced by >1.5 fold by mutant proinsulin expression and mean fold-change compared to control cells from N = 2 independent microarray experiments. Column three is the mean fold-change induction of the same genes in the presence of the IRE1 inhibitor 4μ8c. Red: genes whose >1.5 fold reduction was not affected by 4μ8c. Microarray source files are deposited in GEO data repository (GSE58866).

Format: XLSX Size: 11KB Download file

Open Data