Email updates

Keep up to date with the latest news and content from BMC Cell Biology and BioMed Central.

Open Access Highly Accessed Review

Recent advances in live cell imaging of hepatoma cells

Sandeep Salipalli, Prafull Kumar Singh and Jürgen Borlak*

Author Affiliations

Centre for Pharmacology and Toxicology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany

For all author emails, please log on.

BMC Cell Biology 2014, 15:26  doi:10.1186/1471-2121-15-26

Published: 8 July 2014

Abstract

Live cell imaging enables the study of dynamic processes of living cells in real time by use of suitable reporter proteins and the staining of specific cellular structures and/or organelles. With the availability of advanced optical devices and improved cell culture protocols it has become a rapidly growing research methodology. The success of this technique relies mainly on the selection of suitable reporter proteins, construction of recombinant plasmids possessing cell type specific promoters as well as reliable methods of gene transfer. This review aims to provide an overview of the recent developments in the field of marker proteins (bioluminescence and fluorescent) and methodologies (fluorescent resonance energy transfer, fluorescent recovery after photobleaching and proximity ligation assay) employed as to achieve an improved imaging of biological processes in hepatoma cells. Moreover, different expression systems of marker proteins and the modes of gene transfer are discussed with emphasis on the study of lipid droplet formation in hepatocytes as an example.

Keywords:
Live cell imaging; Fluorescence; Bioluminescence; Green Fluorescent Protein; Proximity ligation assay; Lipid droplet