Phenylalanine incorporation into proteins in myoblasts and myotubes depleted of PDCD4. (A) L6 myoblasts were treated with scrambled (SC) or PDCD4 oligonucleotides as described in Figure 5. Cells were then deprived of amino acids and serum for 12 h, after which phenylalanine incorporation into proteins was measured. (B) Myotubes were treated as in Figure 5 except that siRNA oligonucleotides obtained from Life Technologies were used. Data are expressed as % of SC. Means ± SE of 3–4 independent experiments.
Kakade et al. BMC Cell Biology 2014 15:2 doi:10.1186/1471-2121-15-2