Adaptation of KIND1 cells to feeder free culture system & characterization of feeder free KIND1 cells & embryoid body (EB) differentiation. (A) Bright field images of KIND1 cells cultured on HFF (a) and geltrex (b) Magnification 10X. (c) Cytogenetic analysis of KIND1 exhibiting normal 46, XX chromosome complement. (B) RT-PCR analysis of pluripotency specific gene transcripts (C) Western Blot analysis for OCT4 in undifferentiated feeder free KIND1 cell lysate loaded in triplicate with β actin as loading control. (D) Expression of representative gene transcripts of ectoderm (MAP2), mesoderm (HAND1) and endoderm (MIXL) in Day 7 and Day 15 EBs grown in suspension culture. The expression is with respect to undifferentiated KIND1 cells (set as 1). Error bars represent ± Standard Error of Mean (SEM).
Pethe et al. BMC Cell Biology 2014 15:18 doi:10.1186/1471-2121-15-18