Table 2

Antibodies used for immunofluorescent staining of cultured cells
Target Antibody Dilution
β-tubulin Monoclonal anti-beta-tubuline-Cy3 1/500
clone TUB 2.1 Sigma C4585
Mucin Monoclonal anti-human gastric 1/200
mucin 5 AC clone 45 M1 Sigma M5293
Z0-1 Purified mouse anti-human ZO-1 1/100
clone 1/ZO-1 610966 BD Biosciences
Mouse IgG1 Goat anti-mouse IgG1 AF488 A21121 1/600
Molecular Probes™ Invitrogen
Control Mouse IgG1 negative control X0931 Dako 1/50

Delgado-Ortega et al.

Delgado-Ortega et al. BMC Cell Biology 2014 15:14   doi:10.1186/1471-2121-15-14

Open Data