Figure 2.

1,25(OH)2D3 blocks NF-κB signaling in MRSA-stimulated hMSCs. Human MSCs were co-culture with methicillin-resistant Staphylococcus aureus (MRSA) for 0 min, 10 min, 1 hr and 24 hrs and harvested for cytopalsmic and nuclear proteins extraction. Equal amount of proteins were used for western blot analyses with indicated antibodies (A-B). Human MSCs were preincubated with vitamin D (100 nM) for 24, 48 and 72 hours, followed by methicillin-resistant Staphylococcus aureus (MRSA)-stimulation for 24 hours. Cytopalsmic and nuclear proteins were extracted for western blot analysis with indicated antibodies (C-D). Experiments were done three times and representative blots were shown. NF-κB-p65 present in nuclear protein complexes with VDR, and NR4A2 of hMSCs. Human MSCs were stimulated with or without methicillin-resistant Staphylococcus aureus (MRSA) for 24 hrs and harvested for nuclear proteins extraction. Equal amount of proteins were used for immunoprecipitation (IP) with anti-NF-κB-p65 antibodies and immuno blots (IB) were done with indicated antibodies (E). Experiments were done three times and representative blots were shown.

Maiti and Jiranek BMC Cell Biology 2014 15:11   doi:10.1186/1471-2121-15-11
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