GRASP co-IPs with Dock180 independently of its cytohesin binding activity. A) GRASP truncation constructs used in these experiments. All constructs are myc-tagged at the N-terminus. B) Hek 293 cells were co-transfected with the indicated GRASP constructs and Dock180. Cells were then lysed, and Dock180 immunoprecipitated with M2-flag resin. The immunoprecipitates were blotted with mouse anti-myc and goat anti-Dock180 antibodies.
Attar and Santy BMC Cell Biology 2013 14:9 doi:10.1186/1471-2121-14-9