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Assessment of ORAI1-mediated basal calcium influx in mammary epithelial cells

Diana GF Ross1, Chanel E Smart2, Iman Azimi1, Sarah J Roberts-Thomson1 and Gregory R Monteith1*

Author Affiliations

1 School of Pharmacy, The University of Queensland, Pharmacy Australia Centre of Excellence, 20 Cornwall St, Woolloongabba, QLD, Australia

2 University of Queensland Centre for Clinical Research (UQCCR), Building 71/918 Royal Brisbane and Women’s Hospital, Herston, QLD 4029, Australia

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BMC Cell Biology 2013, 14:57  doi:10.1186/1471-2121-14-57

Published: 20 December 2013

Additional files

Additional file 1:

HC11 cells were treated with siRNA for Orai1 (A.), Stim1 (B.), Stim2 (C.) and Spca2 (D.). Total mRNA was isolated 24 h after siRNA treatment before real-time. RT-PCR for assessment of gene silencing. Data were normalised to Ppib and ActB mRNA, and are shown as relative fold expression to siNT treated HC11 cells (n=4; mean +/- SD, *P < 0.05).

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