Viability and growth of sec61∆L7 yeast under different stress conditions. A) Growth of SEC61, sec61∆L7 and sec61-32 cells was analysed on YPD at 37°C, 30°C (3 d) and at 20°C (7 d). B) Tunicamycin sensitivity was examined on plates containing no (left), 0.25 μg/ml (center) or 0.5 μg/ml tunicamycin (right) at 30°C for 6 d. C) UPR induction in SEC61 wildtype and the indicated sec61 mutant strains was measured as beta-galactosidase activity after transformation of the strains with a UPRE-LacZ plasmid (31) or a plasmid encoding LacZ alone (30). Wildtype cells treated with 2 μg/ml tunicamycin for 1 h and sec61-3 cells shifted to 20°C for 1.5 hrs were used as positive controls. Samples were measured in duplicate and the experiment performed 3 times.
Tretter et al. BMC Cell Biology 2013 14:56 doi:10.1186/1471-2121-14-56