Western blot analysis of oligo-dT-precipitated RNA-protein complexes. (A) RNA-protein complexes form human neural progenitor cells (hNP1) and hNP1 cells differentiated to neurons over 6 days (6dN) were precipitated with oligo-dT beads analysed by SDS page western blot probed with anti-BORIS antibody (ab18337, Abcam). (B) Immuno-blotting of oligo-dT-RNA bound protein complexes from HEK293T cells transiently expressing BORIS (C3-BORIS) or empty vector (C3-empty). Western blot analysis of Input (2%) and oligo-dT bound protein complex probed with either anti- GFP or anti-BORIS.
Ogunkolade et al. BMC Cell Biology 2013 14:52 doi:10.1186/1471-2121-14-52