Depletion of the THOC5 gene causes severe pathological alteration in the gastrointestinal tract. Rosa26-CreERT2 and Rosa26-CreERT2:THOC5 (flox/flox) mice were treated with (+) or without (−) tamoxifen and jejunum (A-E), caecum (E), and colon (E) were fixed in formalin. Paraffin sections were stained by hematoxylin and eosin (HE) (A) or were supplied for immunohistochemical staining using THOC5 (B, E), Ki67 (C), and cleaved caspase 3 (Cl. Caspase 3) (D) specific antibodies as indicated. Bars represent 400 μm (HE) or 20 μm (immunohistochemical staining).
Saran et al. BMC Cell Biology 2013 14:51 doi:10.1186/1471-2121-14-51